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While we found strong effects of temperature on the metabolome, genotype proved to affect an even greater number of metabolites than temperature.
First, the cadmium tolerant genotype proved to be also more tolerant to Microcystis at the organismal level, while the cadmium sensitive genotype was more sensitive to Microcystis stress (i.e., decreased reproduction after Microcystis exposure compared to a control).
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However, subanalysis of the genotype proves that SDHx mutations, although the most prevalent, were not the exclusive aetiology of the metastatic cases included in our cohort.
The addition of only few primer pairs in genotyping proved to yield very detailed haplotype data when compared with genotypic data generated by single-SNP PCR.
Competition assays, in which antibodies were pre-incubated with one or more intact VLPs representing different genotypes, proved useful in further assessment of the antibody specificity detected by LIPS in complex polyclonal sera.
Based on the results, 37 genotypes proved to be self-compatible carrying the SC-haplotype, from which 33 were homozygous and only four were heterozygous (Table 1).
The combined biochemical and bioinformatic protocols achieve a specificity of ~96% when based on 355,000 reads per sample (Table 4, sample indexed by CGCTA; 1 out of 27 positive genotypes proved false).
Although there is evidence of influence of temperature on the soybean seed oleic acid content [ 9, 10], two of our three high oleic acid soybean genotypes proved to be capable of producing a high and stable oleic acid content in three environments.
The control samples, which serve as external controls, are successfully analysed for many times during routine genotyping, proving the reproducibility of the assay.
Should one particular indel region and associated genotypes prove more influential with respect to the incidence of classical BSE, differences regarding overall susceptibility and resistance for B. indicus and B. taurus cattle may be elucidated.
Genotype and allele frequencies of IL12B gene were outlined in Table 2. Successful sequencing of CC genotype was proved by detection of a single peak corresponding to C nucleotide as shown in Figure 1.
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