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Thus perhaps another 20 k genotype assays for the remaining regions would yield a 120 k SNP chip.
In RIL populations, as in the case here, the heterozygous genotype is rare, making the genotype assays easier to score.
All genotype assays used in this analysis were done at the Core Genotyping Facility of the Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, MD, USA.
Genotype assays for polymorphisms in GSTT1, GSTM1, and N-acetyltransferase 2 (NAT2) were conducted at the Core Genotyping Facility of the Division of Cancer Epidemiology and Genetics (National Cancer Institute).
The SNPs were genotyped by pre-designed Taqman genotype assays (C_29002970_10, C_1421536_10 and C_357888_10 respectively, Applied Biosystems Inc, Foster City, USA) according to the manufacturer's protocol using StepOnePlus Real-Time PCR system (Applied Biosystems Inc, Foster City, USA).
From January 1995 to December 1997, we processed 995 buccal swabs for use in polymerase chain reaction (PCR -based genotyPCR -basedin the context of ongenotypeleculassaysdeminlogic stheies.
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Ward, T.J., Ducey, T.F., Usgaard, T., Dunn, K.A. & Bielawski, J.P. Multilocus genotyping assays for single nucleotide polymorphism based subtyping of Listeria monocytogenes isolates.
Genotyping assays for the initial screen were run on the Illumina BeadArray platform (www.illumina.com).
Genotyping was performed using TaqMan predesigned SNP genotyping assays (Applied Biosciences).
After removing failed assays, 34.398 duplo genotyping assays remained.
The genotyping assays used were ViroSeq® and TrueGene®.
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