Exact(19)
The Aptima HPV assay (AHPV) for high-risk human papillomavirus (hrHPV), and the Aptima HPV 16 18/45 Genotype assay (AHPV GT) for HPV16 and for HPV18 and/or HPV45 (HPV18/45) genotypes are approved for cervical cancer screening by the U.S. Food and Drug Administration.
Using 100 clinical serum samples, the performance of the PCR-ELOSA method was compared with Roche's COBAS Amplicor HCV Monitor V2.0 assay and the VERSANT HCV genotype assay (LiPA), and the overall agreement was 99% at the level of HCV genotypes with a detection range of 2.0 × 102 to 1.0 × 107 IU/ml for PCR-ELOSA.
The liquid microarray HCV genotyping test performed as well as the test currently used (Versant™ HCV genotype Assay LiPA).
The HCV genotypes for these samples had previously been determined with the Versant™ HCV genotype Assay LiPA (version I; Siemens Medical Solutions, Diagnostics Division, Fernwald, Germany).
A genotype assay could not be designed for rs689467.
Description and methods for each genotype assay can be found at [ 9, 10].
Similar(41)
DNA extraction and genotype assays of PCR-based markers was as described (Hsu et al. 2014).
Sample selection was based on HCV genotype as had been previously assigned using the Versant™ HCV genotype Assay-LiPA (version I; Siemens Medical Solutions, Diagnostics Division, Fernwald, Germany).
Assessment of the final costs related to LiPA v.1 included the Amplicor® Hepatitis C Virus-HCV-Test, version 2.0 Roche (for PCR product generation) and the VERSANT™ HCV Genotype Assay-LiPA v.1 (Siemens).
Assessment of the final costs related to LiPA v.1 included the Amplicor® Hepatitis C Vírus HVC Test, version 2.0 (Roche) and the VERSANT™ HCV Genotype Assay-LiPA v.1 (Siemens).
For the computation of heterozygote losses/gains, we computed pfalse and qfalse that is, the erroneous values of p and q that are obtained according to the loss or gain (chance or genotype assay-related or genotype-dependent ascertainment-related) of a genotype group dependent on k.
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