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For the analyses in this paper, the loci were chosen to be uniformly spaced across the whole genomic, with a distance of 1 Mb between neighboring loci (leading to a total of 2936 genomic loci in which the PCF function value was determined by the above interpolation formula).
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Cells from different clones were collected to prepare genomic DNA with a genomic DNA extraction kit (Aidlab, China).
The relative dissimilarity is expressed as a percentage of genomic fragments with a lower genomic dissimilarity than the query sequence.
Moreover, it proves useful for identifying genomic markers with a common effect across multiple genomic studies due to its weak dependence on sample size variations.
A genomic scar can be defined as a genomic aberration with a known origin.
Accurate across-breed genomic predictions rely on the presence of common genomic variants with a common substitution effect in each breed.
We first extracted genomic DNA of the cell populations and hybridized the genomic DNA with a biotinylated oligo containing the T7 promoter sequence.
Genome-wide association studies (GWAS) associate genomic variants with a trait of interest to identify positional candidate loci [ 13, 14].
This platform is a high-resolution 60-mer oligonucleotide-based microarray that allows genome-wide surveys and molecular profiling of genomic aberrations with a resolution of ∼75 kb.
The Tu-B primers sequenced genomic DNA with a success rate of 76.47%, and the Tu-D primers sequenced genomic DNA with a success rate of 88.24%.
Furthermore, this genomic region is also of special interest as it represents a GC-rich genomic region with a very high gene content.
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