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Although genome re-sequencing offers the most inclusive possible overview of the genomic variability of small genome species [ 60, 61], methods based on the reduction of genome complexity such as GBS represent a cheaper and versatile alternative to genotype any species of interest in multiplex.
Current high throughput sequencing technologies, together with the recent completion of porcine's genome and its annotation [ 3], makes it possible to study the genomic variability of specific breeds with a detail that was not possible until now.
However, it is believed that the use of 'single cell' genomics (Woyke et al. 2010) could yield extensive information about the genomic variability of a bacterial population and could lead to improved differentiation of bacterial lineages on the basis of their nucleotide polymorphisms and whole genome gene complement.
In addition, we provide evidences that the mobility of these elements has contributed to the genomic variability of this species.
An innovative approach for revealing intraspecific genomic variability of B. cereus and B. licheniformis was the PCR fingerprinting of the spaces between the 16S and 23S rRNA genes and of intergenic tRNA genes regions [60].
The genomic variability of 15 representative field isolates of A. pleuropneumoniae serovars 1, 7 and 15 is shown in Figure 4.
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This method may provide more accurate correlation between clinical outcomes and different genotypes and their subtypes, as well as improved understanding of the role of genomic variability on HCV infectiousness and on its demographic pattern of infection.
The impact of hepatitis B virus (HBV) genomic variability on the measurement of HBsAg level has been poorly evaluated.
To further assess the impact of genomic variability on recombination profiles, additional crosses would be required.
However, the multi-copy nature and intra-genomic variability of the ITS2 [36], [36] often results in the isolation of more than one ITS2 sequence type from an individual Symbiodinium cell, and this interpretational complexity combined with low genetic divergence among ITS2 sequences [e.g. 9] makes the application of this marker in species assignment problematic [16], [37].
The insertion element IS 6110 is an important source of genomic variability for M. tuberculosis and is distributed in different positions across the bacterial chromosome [ 12].
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