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The molecular age of medicine has provided genomic tools that can identify a large number of potential therapeutic targets in individual patients, heralding the promise of personalized treatment.
A major hurdle in obtaining functional data from emerging model systems like Thalictrum, is a lack of transgenic techniques and genomic tools that are readily available for established model plants such as Arabidopsis thaliana.
Microarrays are high-throughput genomic tools that allow the comparison of global expression changes in thousands of genes between different experimental conditions in cell/tissue analysis, and they have been widely adopted for analyzing the global gene expression profiles in vivo and in vitro [10].
In this manuscript we report the development of new genomic tools that will facilitate these discoveries in two Caribbean coral species, Acropora palmata and Montastraea faveolata.
These resources for genetic investigations include functional genomic tools that compare the expression profiles of thousands of genes under different conditions.
Bacterial artificial chromosome (BAC) libraries are key genomic tools that enable positional cloning of important traits, synteny evaluation, and the development of genome framework physical maps for genetic linkage and genome sequencing.
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Next-generation sequencing is a powerful genomic tool that can be used to investigate the transcriptome (the abundance of RNA species), the translatome (the ribosome occupancy on mRNAs) and the interactome (RNA-protein binding).
Another genomic tool that has proven to be beneficial in experimental investigations of enzyme recruitment is the Keio collection, which constitutes ∼4000 nonessential single-gene knockout strains of E. coli K-12.
Numerous studies applying these genomic tools revealed that many RBPs associate with distinct RNA target sets comprised of a few up to several hundred RNAs, which are often enriched for specific sequence/structural elements that define RBP binding sites.
We further discuss the morphological, behavioral, molecular and genomic tools available that together make semi-aquatic bugs a prime model for integration across disciplines.
In conclusion, the RMCE swapping method is a robust genomic engineering tool that offers great potential for accurate genome manipulation.
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Justyna Jupowicz-Kozak
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