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Sequence data obtained from the iMDA template and from both of the genomic templates covered more than 99.98% of the reference genome at least 1X coverage) and greater than 99.9% of the genome with at least 5X coverage.
Single adult sctt and wild type control flies were harvested and used for genomic templates.
qRT-PCR primers (Table S2.4) were tested for specificity in PCR assays using genomic templates.
As a common practice during all PCR analysis, negative controls with no genomic templates were included and were negative.
Retrospective testing of some targets using genomic templates demonstrated concordant results to those observed using synthetic templates.
ChIPs rely on specific antibodies to precipitate crosslinked protein-DNA complexes, followed by quantitative PCR (qPCR) analysis of the enriched genomic templates.
Similar(38)
For each genome scaffold hit, the genomic template including the Ac-DAF-16 bound region and up to 3 kb extensions at both ends were extracted.
We have previously described a method based on random-primed labeling without denaturation of genomic template DNA to map ssDNA genome-wide (Feng et al. 2006).
Genomic template stability calculated through changes in RAPD profile showed consistent results when compared with the indicator and modulator parameters.
Significant differences in band intensity, loss and appearance of bands as well as genomic template stability and band sharing indices of treated plants revealed increased vulnerability of genomic DNA to NiO-NP, at even lowest concentration (10 mg L−1).
In this article, we present an improved OE PCR method that can be used for the generation of large DNA fragments (up to 7.4 kb) where at least 13 changes can be introduced using a genomic template.
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