Sentence examples for genomic target it from inspiring English sources

Although, the majority of the BC1 lines displayed excised genomic target, it is difficult to give a precise quantitative assessment of the phiC31 activity since only a modest number of different target locations were thoroughly characterized.

In the case of gene targeting with ZFNs, once a pair of nucleases has been established for a given genomic target, it would be useful to be able to introduce sequence changes at various sites within that locus without the need to develop new cleavage reagents specific for each site.

Although, the majority of the BC1 lines displayed excised genomic target, it is difficult to give a precise quantitative assessment of the Bxb1 activity since only a modest number of different target locations were thoroughly characterized and direct comparison to other recombinases was not addressed.

When assaying genomic targets, it is very useful to have fluorescence based methods, which are not dependent merely on relative strength of emission intensities, but which also encompass rates and direction of change of emissions over time.

Subsequently, researchers turned to the use of group II intron retargeting systems in which an intron-encoding segment of DNA could be deliberately inserted into the genomic target from where it could not excise [ 11– 13].

The ability of CRISPR-it to efficiently reveal recessive mutant phenotypes associated with a large proportion of genomic target sites also makes it a highly attractive system for large scale F1 mutagenesis screens.

The Cas9 enzyme is guided to its genomic target sequence by a small guide RNA with a complementary sequence.

We are currently combining this platform with deep sequencing to understand how transcription factors find their genomic targets, and adapting it for high-throughput enzymology measurements with the goal of improving enzyme design.

Each array type is also associated with a manifest file (with file extension BPM or BGX) that provides details of probe sequences, intended genomic targets and whether it is a control probe or not, information that is necessary to correctly interpret the data.

Moreover, it is unclear what proportion of genomic target sites can be modified with high efficiency.

These findings can be extended to other methods for pooled genetic screens by noting that it corresponds to ∼1400 reads per genomic target per condition.