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Similar results have previously been reported [13], [14], implying that highly conserved host-like genes are not uncommon in environmental phages and therefore that the total diversity of the global phage genomic pool could be significantly smaller than an assessment of phage gene content would indicate.
It provides insights into the genomic pool of microorganisms that are recovered directly from environmental sources.
The Cy-5 test signal was divided by the control signal (genomic pool) and each microarray was normalized to the mean of the control group.
The beads were washed once with 200 μL hybridization buffer (5 × SSC, 0.1% SDS) for 5 min, then 150 μL of the denatured genomic pool were added to the resuspend the beads.
One hypothesis suggests that these rare genotypes (if real) may represent a large genomic pool, which helps the protistan community to react to any biotic or abiotic changes [ 43].
In B. grandii, the pattern of variability of Bag530 and 3'IGS-ETS might be explained by bisexuality acting as a driving force on repeated sequence homogenization/fixation in the same genomic pool (molecular drive), coupled with the co-evolution of the rDNA arrays within each gene pool.
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This study illustrates that the massive parallel sequencing of genomic pools is a cost-effective approach to identify footprints of selection.
Additionally, Amaral et al.'s (2011) study showed that sequencing of genomic pools by next-generation sequencing was a cost-effective approach to identifying selection signatures without the effect of ascertainment bias.
Genomic pooled normal control DNA and DNA from LNCaP or from 22Rv1 cells were fluorescently labeled by random priming and were hybridized to RPCI arrays containing ~6,000 RPCI-11 BAC clones, as described [ 11].
Equal amounts of DNA of each of the four pools was pooled together to obtain the genomic DNA pool for sequencing.
An equal amount of DNA from each of the four pools was combined to obtain the genomic DNA pool for sequencing.
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