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The next step after read quality improvement is to map the obtained reads against a reference genome to infer the genomic origin of a read.
In this study we first determine whether the α-gliadin genes present in the A-, B- and D-genome ancestral species are sufficiently different to attribute the ancestral genomic origin of the α-gliadin genes in hexaploid bread wheat.
We also assessed if the genomic origin of MP reads were biased towards different fractions of the genome (i.e. repeat or conserved fraction).
The sequences were compared to human DNA to determine the genomic origin of the small RNA.
Using GISH, the genomic origin of the chromosomes could be resolved, although the distinction was less clear than at metaphase.
The copy number and genomic origin of any marker system, and the potential for redundancy between markers, is an important consideration for evolutionary applications.
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These reads were mapped to the N. crassa reference genome assembly 12 (AABX00000000.3) to identify genomic origins of small RNAs.
As a result, what we have is a set of reads that are most similar in sequence to their respective mapped locations in the reference genome, which are presumably the genomic origins of the fragments that produced the reads.
Using the SNP data sets from Tongil and its parents, we defined the genomic origins of regions of the Tongil genome by SNP calling (Additional file 3: Figure S1; Additional file 4: Table S3; see also the SNP calling section in the Materials and Methods), and then performed a SEG-Map analysis (Zhao et al. [2010b]) of Tongil (Figure 2).
In this design, the genomic origins of the reads can be identified.
Data produced can be helpful to investigate the genomic origins of phenotypic variation as well as to perform breeding programs.
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