UTILLdb is an open phenotypic and genomic mutant database containing information on mutant populations of pea [ 37], Brachypodium [ 52], tomato [ 53] and flax (this paper).
Interestingly, a recent survey on classical mutations and genome polymorphisms in N. crassa [ 36] revealed significant genomic variation in classical mutant strains in this fungus.
HAT-RAPD (High Annealing Temperature-Random Amplified Polymorphic DNA) was applied for analysis of genomic variation in the mutant.
The P. aeruginosa PUPa3 WT strain and all QS genomic null mutants have all been described previously (Steindler et al., 2009).
Using 123 genomic insertional mutants generated by random transposon mutagenesis [16], we performed a phenotype screen for reduced in vitro parasite proliferation rates in the parasite blood stages.
GROMO contains information on genomic sequence, mutants available, gene expression, localization of proteins obtained from a variety of repositories, as primary data.
The model correctly predicts the behaviour of genomic knockout mutants in which the QS genes responsible either for the synthesis (lasI, rhlI) or the sensing (lasR, rhlR) of AHL signals were inactivated.
We have generated, for the first time, a random genomic insertion mutant library for a halophilic archaeon and used it for efficient gene discovery.
However, the original diversity of the plasmid library could not be maintained in the final H. volcanii genomic insertion mutant library.
A practicable strategy to analyse genes of unknown function is to mutate them, e.g. by generating a genomic insertion mutant library, and subsequently characterize the phenotypic properties of individual clones within the library.
