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Established targeted insertion with right direction was confirmed at the genomic level with PCR (Fig. 1D).
Established targeted insertions were confirmed at the genomic level with PCR for all six lncRNAs as described above (Fig. S3B).
Established targeted insertions were examined and confirmed at the genomic level with PCR for all six lncRNAs similarly as described above (Fig. S4B).
This protein is encoded by a 1668 bp cDNA corresponding at the genomic level with a single-copy 4 kb gene that is exclusively transcribed in the tsetse salivary gland tissue.
In the last decade the field of aDNA has moved from the genetic to the genomic level with the advent of massively parallel sequencing platforms.
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We found evidence for the existence of such gene families for 8 of 9 sequences tested; in addition to intra-specific differences among strains, there was considerable intra-strain variability on both the transcriptomic and genomic levels, with variation in the cDNA within a strain, comprising both synonymous and non-synonymous differences.
This agrees with recent findings on how mutation at the genomic level, combined with neutral evolutionary forces, shape emergent properties at the network level (Ruths and Nakhleh 2013) and can explain correlations between network properties and gene duplicability (Zhu et al. 2012).
At the genomic level, together with the mutation/modification of regulatory elements, three driving forces are instrumental for the diversification.
Clearly, meta- and synchronous tumors show differences at the genomic level incompatible with a simple genetic progression from initial to recurrent tumors.
Although no molecular identity has been ascribed to the mitochondrial KATP channel at the genomic level, similarities with the components of the plasma membrane KATP channels have been proposed.
Established targeted insertion and lncRNA tagging were confirmed at the genomic level and mRNA level with PCR/RT-PCR, respectively (Fig. 1B).
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