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The genome of S. aureus was extracted by Genomic Extraction Kit (Qiagen, Valencia, CA, USA) and applied as the template for amplifying Δ59-srtA with the primer pairs: CAT GCC ATG GAA GCT AAA CCT CAA ATT CCG; and CGC GGA TCC TTA GTG GTG GTG ATG ATG ATG TTT GAC TTC TGT AGC TAC AAA GAT.
Genomic DNA from cell lines was extracted using a genomic extraction kit (Sigma).
Genomic DNA from cell lines was extracted using a genomic extraction kit (Gentra System, Minneapolis, MN, USA).
DNA was extracted using GeneAll Exgene Mini genomic extraction kits (GeneAll, South Korea) following the manufacturer's instructions.
In our data, the sequencing coverage on the X chromosome would be expected to be only be three-fourths of autosomal coverage because the whole-genome shotgun sampling was from a mixed sex (50 50) genomic extraction.
DNA was extracted from each quinolone-resistant isolate, using the Promega Wizard genomic extraction kit.
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Complete genomic extractions were performed using QIAgen QIAamp tissue kits (QIAGEN, Valencia CA).
Liver sections were snap-frozen for genomic DNA extraction (Qiagen DNA extraction kit, Qiagen, Belgium).
DNA was extracted with Plant Genomic DNA Extraction Miniprep Kit (Viogene, Taiwan), according to manufacturer's protocol.
DNA was also extracted using a genomic DNA extraction kit (G-500, Qiagen).
Bacterial genomic DNA extraction was done using the ZR Soil Microbes DNA Mini-Prep extraction kit (Zymo Research Corporation, South Africa).
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