Symbol L was proposed for tomato (section Lycopersicon) on the bases of preferential chromosome pairing and clear-cut parental genome discrimination by using genomic in situ hybridization (GISH) and LLEE or artificial amphidiploids of tomato and S. etuberosum [ 11].
Similar to Nicotiana allopolyploids, the efficiency of genome discrimination decreases in the cases of tetraploid and hexaploid wheat, likely due to increased cross-hybridisation of the BAC_2383A24 (B-genome) repeats and Ae. tauschii genomic DNA with chromosomes from homoeologous genomes.
The scope is unprecedented, ranging from genomic-based discrimination between causative agents (7) to increasingly comprehensive global epidemiological data (8).
This approach could be extended along many investigation lines, namely exported in other contexts of computational genomics, as a basis for discrimination of genomic pathologies.
Instead, the concern is mostly focused on discrimination against genomic DNA contamination and/or carry-over DNA, which can give a false-positive PCR product [ 19- 21].
It has allowed for the discrimination of genomic changes, differential expressions and subsequent signaling effects for a variety of proteins in diagnostic tissues [ 4- 10].
A polymorphism in the human SMAD7 gene (NM_005904) associated with increased risk of developing colorectal carcinoma (rs4939827) was used as a model system to study the performance of rhPCR for SNP discrimination with genomic DNA.
To evaluate whether the method proposed by Nwakanma et al. [ 20] would enable the discrimination of genomic constitution and ploidy, preliminary assays were carried out using mixtures of DNA samples from the M. acuminata ('Calcutta 4') and M. balbisiana ('Butuhan') genomes to obtain various artificial combinations of genomes, mimicking the natural ones.
Regulation will be required for comprehensive protection of persons from genetic discrimination that may result from the abuse of sensitive genomic information.
Graft levels of the immune system were quantified by using a TaqMan allelic discrimination assay on whole-spleen genomic DNA, with dilutions of genomic DNA of the respective homozygous Mcs5a genotypes as standards.
