Sentence examples for genomic constructs were from inspiring English sources

Genomic constructs were prepared by maxi-prep (Qiagen, Germantown, MD, USA) and injected into BL5905 (w 1118 ) (Genetic Services, Cambridge, MA, USA), and transgenic flies were selected and maintained over the TM6C,Sb balancer chromosome.

The complementation genomic construct was generated by insertion of a 3747nt-long PCR amplified genomic product from 868 bp to the stop codon into the NotI site of pCBI [52].

In order to document the subcellular localization of DRB2, the above-mentioned DRB2 genomic construct was fused to the coding sequence of GFP (Green fluorescent protein) and bombarded into onion cells.

The UAS- lrrk, UAS- lrrkGS and CaSpeR- lrrk genomic rescue constructs were previously described (Dodson et al., 2012).

Genomic PIN1 constructs were prepared by insertion of a 3558 bp Sal1/ Not1-digested PCR fragment including the PIN1 open reading frame and terminator into pGREEN0229.

To this end, each CNS was amplified by PCR on mouse genomic DNA and chimeric constructs were generated inserting each fragment in the pGL3-basic reporter vector containing the E1b minimal promoter upstream of the firefly luciferase.

Measuring hepatitis C virus (HCV) replication capacity utilizing primarily sub-genomic reporter constructs is limited.

The circularized constructs containing the genomic DNA were column-purified and sheared using Adaptive Focused Acoustic technology (Covaris, Inc .. Sheared constructs were end-repaired and ligated with 454 paired end sequencing adapters.

As shown in Figure 1a, the D76A point mutation was engineered into a myostatin genomic construct, which was used for homologous targeting in embryonic stem cells.

A genomic RPS10B construct was produced by amplifying a 3.5 kb fragment spanning the RPS10B genomic region from Columbia wild-type genomic DNA with primers RPS10BgenomicF and RPS10BgenomicR (Table  5).

The predictive ability from SNPs in CDS was better than those for Genes1kb-IGR even though the two additive genomic relationship kernels constructed were from only 14,416 SNPs and 184,047 SNPs, respectively.