Sentence examples for genomic construct from inspiring English sources
Primers 3 and 4 were used to identify homozygous knock-out mutants rescued by the full genomic construct.
The guard cell expression of AHK5 was confirmed by creating an AHK5 promoter-GFP-AHK5 genomic construct (PAHK5 GFP-AHK5) and transiently expressing this in tobacco leaves.
As shown in Figure 1a, the D76A point mutation was engineered into a myostatin genomic construct, which was used for homologous targeting in embryonic stem cells.
Rescue by the genomic construct indicates that all cis-acting control sequences required for functional Ntl expression lie within 2.7 kb upstream (distal) of the gene.
A pCaSpeR 4 genomic construct extending from 2.7 kb upstream of the Ntl transcript to 519 bp beyond its 3'end also rescued the Ntl mutant phenotype (4 lines; all rescued).
This pattern was reminiscent of the expression pattern of Dnmt2-EGFP expressed from the genomic construct (Fig. 4D,E) suggesting that cycling neuroblasts allow access of the transactivator fusion protein to DNA.
The phgPrP-myc plasmid, driven by the endogenous Prnp promoter in the context of the PrP "half-genomic" construct (phgPrP) [14], was digested with NotI and SalI to remove its prokaryotic backbone.
To test this hypothesis, we introduced two point mutations at codons 232 and 233 (original mouse numbering) of the half-genomic construct PrPΔCD [10], resulting in two in-frame stop codons.
Next, Tg mice were created using a 'half-genomic' construct expression vector derived from the mouse Prnp locus (Borchelt et al, 1996; Fischer et al, 1996).
To investigate LHP1 binding in the lif2 mutant, we generated a genomic LHP1 MYC construct; this genomic fragment had been previously used for lhp1 complementation [26].
To examine the role of the Grb2/Drk-binding site in fly physiology, we generated flies carrying this mutation as a genomic rescue construct alongside genomic rescue constructs containing the wild-type chico sequence as well as a second construct with mutations to disrupt binding of Chico to the p60 subunit of PI3K.
