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For the whole-genome resequencing workflow, finally, the software uses BWA for genomic alignment and calls single nucleotide polymorphisms with SAMtools [ 13] and structural variations with Delly [ 16].
Sequence common to the GAS genomes constituting the endogenous metagenome core, were identified by a combination of genomic alignment and gene content comparisons.
JCVI (J. Craig Venter Institute); JGI Joint Genome Institutee) The five genomes were compared using Artemis Comparison Tool (ACT) [ 15], which enabled genomic alignment and visualization of BLASTN results.
The absolute genomic coordinates of the integration sites where defined as a result of the combination of genomic alignment and vector relative orientation data.
This change simplified genomic alignment and, more importantly, enabled single-nucleotide resolution of retrotransposon integration sites.
Genomic alignment and SNP detection were carried out as described in Methods.
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Genomic alignments and PhastCons scores were downloaded from the UCSC genome browser website [ 14, 15].
To determine the ease of cross-species comparison, genomic alignments and putative orthologues were searched for in both human and rat genomes compared to mouse alpha-defensins.
The algorithms that generate genomic alignments and transcript annotations differ for the three platforms.
Genomic alignments and base-pair numbering relate to Build NCBI m37, Ensembl release 59.37 L of the mouse genome.
REDHORSE is an efficient analytical pipeline that serves as a bridge between genomic alignments and existing recombination detection algorithms.
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