One important option to get rid of complexity is to create a so-called 'minimal genome' that can function as a basic biological framework or 'chassis' into which new, designed and synthesized segments or sequences that encode novel, economic useful functions can be implemented.
PO activity was easily detected in crude hemolymph from fourth- to fifth-instar honey bee larvae but there are at least two gene products in the honey bee genome that could function in this assay based on domain comparisons [ 22].
The silent elements and other natural genetic engineering agents do not disturb a genome that is functioning well.
We show that the dinoflagellate host genome expresses genes that function in several plastid-associated pathways, and that homologous genes are also expressed in the endosymbiont.
These results provided strong support for the idea that purifying selection has removed the phenotype-modifying allelic variance from the mitochondrial genome that affects female function, but has missed a class of mutations that are male-biased in their effects.
By further performing the Rnr3 R-SGA screen in the presence of MMS (an exogenous source of DNA damage), we aimed to isolate genome maintenance factors that function specifically in tolerance of MMS damage.
This can be dealt with however, by examining the question of interest within ethnic groups, separately analyzing the different sub-populations, and/or adjusting for principal components from genome wide data that function as ancestry markers, relying on the conditional independence assumption.
To eliminate the complexity of the FokI and nickase-based platforms while retaining the potential for high specificity, we recently developed novel genome-editing reagents that function as monomers by fusing the small, sequence-tolerant nuclease domain of the GIY-YIG homing endonucleases (GIY-HEs) to customizable DNA-binding proteins (Kleinstiver et al. 2012).
Based on a homology search, we identified two RBPs from the P. falciparum genome that have putative functions in genome maintenance.
