Sentence examples for genome polymerase from inspiring English sources

To test for alternative recombinogenic conformations of the mitochondrial genome, polymerase chain reaction (PCR) was carried out using total genomic DNA and primers designed by Primer3 [ 68] in Geneious R6 (Additional file 1: Table S12).

To determine the integration of cassettes in the genome, polymerase chain reaction (PCR) was conducted using specific primer pairs (p35: 5'-cccacagatggttagagagg-3' and 5'-gtagtagtcgttgcgttcgt-3' and op-iap: 5'-atgagctcccgagcaatt-3' and 5'-acgcctcagtcatcaccc -3') to amplify the CaMV 35S promoter p35 region and op-iap from T1 to T3 transgenic cotton plants.

The RFLP profiles of isolates in the noncapsid region, at the 3′-terminal end of the genome (polymerase 3D and 3′ noncoding regions: nucleotides 6,535 to 7,439) also confirmed the presence of two separate groups.

Putative S/MARs were amplified from the G. lamblia genome by Polymerase Chain Reaction using Taq polymerase (NEB, USA).2 ng G.lamblia genomic DNA was used as a template.

Each Salmonella effector gene was cloned into the eukaryotic expression vector pIRES2-EGFP either by subcloning the gene from another plasmid or by amplifying the gene from the Salmonella genome by polymerase chain reaction using Deep Vent DNA polymerase (NEB).

The congruence of the genome and polymerase trees suggests that a large set of viral genes, including polymerase, derive from a phylogenetically conserved core of genes of host origin, secondarily reinforced by gene acquisition from common hosts or co-infecting viruses within the host.

First, Taylor and Bruenn unequivocally proved that there is integration of dsRNA viral genomes into the fungal genomes by polymerase chain reaction analysis of the boundaries between the integrated virus genome copy and the host DNA; the identification of the chimeric sequences leaves no doubt that the virus-specific sequences are, indeed, integral to the fungal genome.

Anterior chamber tap was positive for MPB 64 genome by polymerase chain reaction.

Strikingly, in a recent paper published in Science (Liu and Cheng 2015), Drs. Liu and Cheng managed to discern the full genome and polymerase of cytoplasmic polyhedrosis virus (CPV), using their new approach adapted from traditional single-particle analysis of icosahedral viruses.

In order to solve this problem, we developed a new symmetry-mismatch reconstruction method and for the first time we determined the structures of genome and polymerase within an icosahedral dsRNA cypovirus (Liu and Cheng 2015).

Each clone was analysed for vector integration in the genome by polymerase chain reaction (PCR; data not shown).