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These analyses revealed three regions that are markedly different in composition compared to the rest of the genome (Figures 1& 2).
Our reconstruction experiments (Figure 5) indicated that the R-dependency be mainly ascribed to selection against cells with the parental genotype in the R+ culture, likely through attack on their genome (Figures 1A and B (ii)).
This was true for both intra-chromosomal and inter-chromosomal gene pairs, indicating that the trend is not due to proximity in the linear genome (Figures 6A,B, respectively).
These peaks also spanned significantly wider in the mid-third of genes than in introns, regulatory regions, replication origins that are known to be AT-rich and therefore less likely to coordinate nucleosomes [30], and the rest of the genome (Figures 4 7, S1, S2, S3, S4, S5).
Genome figures were made using Circos [ 36] and Mauve.
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PCR results indicated that the 150-kb whole-genomic hLF sequence was introduced intact into the bovine genome (Figure 1A).
With this approach, the SNV agreement between FF and FFPE samples increased from 52 to 63% when screening the whole genome (Supplementary Figure S12C) and 71% when selecting variants in reliable regions (Genome in a Bottle regions, representing 69% of the genome) (Figure 2d).
We developed a recombinant PCR method for in vitro creation of linear constructs for the replacement of every protein-coding gene in the S. sanguinis SK36 genome (Figure S1A).
The genetic factors conditioning BT-, HL-, and WA-CMS are all chimeric genes, probably as a result of the rearrangement of the mitochondrial genome (Figure 1).
The total number of DNA polymorphisms was 1,154,063 including 1,024,202 SNPs, 53,180 insertions and 76,681 deletions between the five accessions and the Nipponbare genome (Figure 1b).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com