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The recent emergence and application of engineered endonucleases have led to the development of genome editing tools capable of rapidly implementing various targeted genome editions in a wide range of species.
Genomes of the Burmese python, Oikopleura dioica, Branchiostoma floridae, Saccoglossus kowalevskii, Strongylocentrotus purpuratus, Aplysia californica, Tribolium castaneum, Bombyx mori,Caenorhabditis briggsae, Loa loa and of the groups Kinetoplastida including Trypanosoma and Fungi were searched using the NCBI browser (http://www.ncbi.nlm.nih.gov/; 2011 genome editions).
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Whole exome sequencing of iPSC before and after CRISPR-Cas9 genome edition revealed no major exonic off target effect of the CRISPR correction.
The current tools for this biotechnology is based in genome edition, allowing adding, repairing or deleting gene information and phenotypic traits as never achieved before.
We are now living the irruption of the third technological wave in which genome edition is possible by using endonucleases, particularly the CRISPR/Cas system.
We illustrate the application of our new marker by significantly accelerating the protocol for genome edition using CRISPR/Cas9 in S. pombe.
In the following decade, genome edition will continue expanding our technical possibilities, which will contribute to the advancement of science, the development of clinical or commercial applications, and the improvement of people's life quality around the world.
Quality trace data were clustered using LaserGene Seqman, Genome Edition (DNASTAR , Inc.
Site-specific deletions and insertions in the chromosome of E. coli were done with the marker-less strategy of genome edition based on expression of I-SceI endonuclease.
Site-specific deletions and insertions in the chromosome of E. coli were originated using a marker-less genome edition strategy based on the generation of double-strand breaks in vivo with I- SceI endonuclease.
GenomeMatcher and In Silico Molecular Cloning Genomics Edition (IMC-GE) software (In Silico Biology, Japan) were also used for intra- and inter-QU 25 genome comparisons.
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