Sentence examples for genome disruption from inspiring English sources
This latest tool in genome editing allows for specific genome disruption and replacement in a flexible and simple system resulting in high specificity and low cell toxicity.
Critical reagents and information were provided by FLYBASE, the Bloomington Drosophila Stock Center, and the Drosophila Genome Disruption Project.
Common laboratory strains Oregon-R and Canton-S were used, as well as an isogenized w1118; Canton-S strain created as part of the Berkeley Drosophila Genome Disruption Project [37].
In addition, off-target genome disruption, as for RNAi, will need to be considered.
Nonetheless, Cas9 and ZFN-mediated genome disruption strategies have unfolded complications owing to the in-frame skipping of targeted exons.
On the other hand, homologous recombination can be utilized to deliberately introduce stop codons and selectable markers for ensuring genome disruption (Additional file 1: Figure S1).
It is widely accepted that HPV-mediated cervical carcinogenesis proceeds via the integration of viral genome and disruption of the E2 ORF, thus releasing the suppressive control of E2 on the expression of viral oncogenes E6 and E7.
Tumor progression is characterized by (1) increased expression of E6 and E7 genes of high risk HPVs, known to bind to and inactivate p53 and pRb oncosuppressors, respectively; (2) integration of viral DNA into host genome, with disruption of E2 viral genes and host chromosomal loci; and (3) molecular alterations of key regulators of cell cycle.
Although P-elements have once been considered a powerful tool for genome-wide gene disruption, single P-element insertions are only able to disrupt 25% of Drosophila essential genes and about 40% of the total annotated genes, according to the estimation from the Berkeley Drosophila Genome Project (BDGP) [ 30, 31].
Recently, we reported a genome-wide disruption of hippocampal 5hmC in male mice following acute stress that was correlated to altered transcript levels of genes in known stress related pathways.
Upregulation of SMARCA1 in Golden Retrievers with hemangiosarcoma was intriguing since changes in expression of a single transcriptional regulator can create genome-wide disruption of a variety of genes, possibly resulting in faster progression of the disease.
