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Additional in silico analyses of whole genome ChIP sequencing (ChIP-Seq) data using the UCSC Genome Browser showed that RNA polymerase II and the c-MYC proto-oncogene are bound to the conserved region preceding the AKT1m exon in MCF7 breast cancer cells [ 17, 18].
In general, our results are in a good agreement with those generated by genome chip technology.
One probe pair in LE could have been hybridized to the same Incyte Human Genome chip several times.
The samples were hybridized to the rat genome chip RG_U34A, and washed and stained according to protocols indicated by Affymetrix.
In rice, surveys using a whole genome chip identified mycorrhiza-responsive genes and revealed a partial overlap in the transcriptional responses to mutualistic and pathogenic biotrophic fungi [ 35].
We set out to develop a genome chip for discrimination of bacteria without prior knowledge of gene targets or specific probe design.
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Affymetrix had total product sales in 2002 of about $250 million, and a company spokesman said that human genome chips are its top-selling product.
Two groups have used MCF7 cells to perform whole genome ChIP-on-chip for ERα location analysis, revealing between ∼1600 3700 receptor-bound loci in response to E2 (with considerable reproducibility) [31], [44].
To address this question, we carried out whole genome ChIP-chip studies to identify all of the Ubx bound regions in the haltere and T3 leg imaginal discs, which are the precursors to these adult structures.
For interpretation of whole genome ChIP-chip datasets in the K562 cell line in the context of gene expression, data was considered for only those genes consistently called either absent or present by MAS5 on Affymetrix Expression GeneChips and which also had ENSEMBL identifiers (Supplementary Table S9).
Consistent with this view, a recent study in mouse ES cells using a whole genome ChIP-seq analysis shows that L1 elements and their flanking regions are indeed enriched with specific repressive histone modifications that distinguish LINE-rich chromatin domains from other gene-rich domains [36].
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