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To test whether Pi starvation affects genomic DNA methylation in rice, whole genome base resolution profiling of DNA methylation by MethylC-seq was performed on rice roots in triplicate at each time point.
Different species possess different GC content at the third codon position (GC3), which largely decides genome base composition.
This dependence of codon usage on genome base composition (AT- or GC-richness) has been previously reported in several unicellular genomes.
From the total 1,445,873-bp reference wRi genome, base calls were made for 1,194,063 bp (82.6%) for the sample of wAnaINF genomes (table 2).
The TopHat alignments were analyzed by coverageBed (BEDTools v.2.16.2 [ 48] to count the number of reads per genome base location.
Data analysis including alignment to hg19 human reference genome, base calling, and identification of variants was done using the Ion Variant Caller (version 2.2).
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The assembly covers 90.2% of the nuclear genome based on our estimation.
c, Simulation of the pan-genome and core genome based on 500 randomizations of rice genome orders.
In particular, subtle differences due to genetic variants are not considered in reference genome based off-target site prediction.
The remaining clean reads were mapped to P. agarivorans Hao 2018 genome based on a local alignment algorithm using Bowtie2 software30.
N'Diaye, A. et al. Construction of an integrated consensus map of the apple genome based on four mapping populations.
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