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The seven S. pistillata genets were used in two sets of experiments.
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Genetic parameters estimated in another paper (Zhang et al., Genet. Sel. Evol. 32 (2000) 41-56) were used to perform the analyses.
Lentiviral pLVX-Teton-Genet vector (Clontech) was used to stably express the tetracycline-controlled transactivator.
Species were identified using haploid diploid pairings and genets were determined using intraspecific somatic incompatibility tests.
The genetic relatedness (rxy: [Ritland, K., 1996. Estimators for pairwise relatedness and individual inbreeding coefficients. Genet. Res. 67, 175 185.]) was used to identify the unrelated pairs wherein a minimum value of rxy for half-sib family (rxy = 0.07) was considered as a critical value of unrelatedness.
All of the ramets from two of the original 20 genets failed to resprout and therefore could not be used in this study.
Then, 2-D non-metric MDS Multi Dimensional Scalingg) algorithm was used on the Bray-Curtis similarity matrix to see whether the new colonies were grouped together according to the genet factor or according to the fragment shape (experimental group).
More than 90% of the damaged genets were sprouting.
The number of sprouts in a non-damaged genet was determined by intrinsic sprouting ability, and the number of sprouts in damaged genets was determined by stump size.
Genets are short-lived (2-3 yeand) and populations are characterized by a high turnover-rate [ 11].
Therefore, we assumed that GeneTs are primarily regulated by tele-enhancers.
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