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The outcrossed pollinations were performed by collecting pollen in a microcentrifuge tube from at least five different genets using a buzz-pollination device (a modified electric toothbrush); vibrating the tube to thoroughly mix the pollen; and then touching the mixture to a stigma.
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Parentage analyses were performed based on the multilocus SSR genotypes of the offspring and candidate parents (i.e. adult genets) using CERVUS version 3.0 [ 50] with maximum-likelihood algorithms [ 51].
Two of the 18 genets used in this experiment did not produce enough flowers to complete all pollinations and were therefore excluded from this study.
Genomic DNA was extracted from leaves of adult genets and seedlings (hereafter referred to as offspring) using a modified CTAB (Cetyltrimethylammonium bromide) method [ 47] with further minor modifications.
Gene expression profiles in dominant and subordinate animals were compared using a "universal" reference design [Churchill GA (2002) Fundamentals of experimental design for cDNA microarrays. Nat Genet 32 (Suppl):490 495].
Nowhere in his text does Genet use the "c" word.
This method uses a graph permutation algorithm to create a null distribution that takes into account connectivity biases of the known interactome to identify genet sets with statistically enriched interactions between RNAi targeted gene products.
The seven S. pistillata genets were used in two sets of experiments.
Paternity analysis was performed by a likelihood-based approach based on multilocus genotypes for all adult genets and offspring using CERVUS version 2.0 [ 41].
As the ladies flung the "c" word around, I tried to remember whether Genet ever used that sliming word in his dialogue, and that's when I ran up against another problem: the translation.
Whole blood collected in a microtube containing 20 μL 7% EDTA are processed using Genet Bio DNA Isolation Kit (PrimePrepth, South Korea) following the procedures detailed in the kit to extract genomic DNA.
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