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This manuscript provides both a novel, and potentially extremely useful new GEVI, as well as important insights into the behavior of genetically expresses voltage-indicators.
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About 2700 copies of genetically expressed peptide copies give superior selectivity and sensitivity to M13 phage-based SPR sensor.
Their system has demonstrated excellent selectivity and sensitivity in the SPR signal by ~2700 copies of genetically expressed peptide on the pVIII major coat protein [64].
We compare biophysically and energetically the cellular responses to direct electrical current injection versus optical stimulation mediated by genetically expressed light-sensitive ion channels, e.g., Channelrhodopsin-2 (ChR2).
To assess growth conditions in which the hyq operon was genetically expressed, A. caulinodans strains using β-glucuronidase activity to report hyq operon transcription were constructed.
The disease is genetically expressed by an extension of the CAG-repeat length at the 4th chromosome [1] encoding a large protein, the "huntingtin".
For the Ca2+ dependent CRET reaction to occur, the genetically expressed mtGA must be reconstituted with the aequorin substrate, CLZN (mtGA-CLZN).
In the present study, we made a comparison of the low-pH stability of sialidase activity between 1918 pandemic and epidemic virus N1 NAs by measuring sialidase activities of cells genetically expressing NAs.
Classical FRET imaging techniques for in vivo applications have been limited to the detection of intramolecular FRET interactions using genetically expressed biosensor constructs [ 19– 22].
We demonstrate here that it is possible to apply OPT to live zebrafish embryos labeled with genetically expressed fluorescent proteins up to at least ~3 days post-fertilization.
In conjunction with advances in labeling technology, particularly genetically expressed fluorescent proteins [ 1], this progression to HCA has driven the development of quantitative fluorescence microscopy techniques.
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