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The 6MP-resistance allele is found in the silent germline nucleus of CU428, but not its somatic nucleus; therefore, resistance indicates the production of true genetic progeny.
See also Figure 6 figure supplement 1. (E ) Percentage of genetic progeny resulting from mating of wild type lines vs isogenic ΔEZL3 lines.
For genetic progeny analysis, the seeds of each field-collected fruit were sown separately in 2008 in a greenhouse at the Kunming Botanical Garden, Kunming, Yunnan, China (25°08'42″ N, 102°44'31″ E).
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In summary, selection of parents reduces the variance of BV (i.e., additive genetic variance) in progeny but in particular the variance of EBVP in progeny.
The genetic diversity in progeny was estimated to be 0.67, i.e. 16.25% lower than the genetic diversity of adult trees (which was calculated to be 0.80).
RNA sequencing-mediated transcriptome analysis of the Kitaake wild type, transgenic plants carrying XA21 (Xand) (23A-1-14), and transgenic plants overexpressing XB21 in the XA21 genetic background (XB21ox/XA21, progeny of 3A-11).
Three replicates, with each replicate of three leaf tissues, were collected from five-week-old Kitaake, transgenic plants carrying XA21 (XA21, 23A-1-14) and transgenic plants overexpressing XB21 in the XA21 genetic background (XB21ox/XA21, progeny of homozygous 3A-11 line), with three replicates.
If the extraordinary expression of hermaphroditism in willows has a genetic basis, the progeny will inherit the hermaphroditic expression, and the sex ratio will depend on the segregation of parental alleles.
In order to simplify interpretation of results, cloned DNA derived wild type DEN-4 was used for comparison of progeny genetic stabilities from passages of the virus in Vero cells and in MRC-5 cells.
Crosses involving high temperature treatment of genetic all-female progeny [ 21] would have been more informative.
To validate the association of ASE SNPs with MD genetic resistance, a progeny test was performed.
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