Sentence examples for genes without the from inspiring English sources

In contrast to QuickChange™ site-directed mutagenesis, which introduces single mutations or small insertions/deletions, RF cloning inserts complete genes without the introduction of unwanted extra residues.

Recently, we developed a simple and efficient method involving biomass-inducible chromosome-based expression system (BICES) for expressing foreign genes without the use of plasmids or expensive inducers (Akita et al. 2015; Nakashima et al. 2014).

Although it is possible to transcribe genes without the corresponding translation (Houben et al. 2009), in the context of our research, the probability of this occurring in five genes simultaneously is extremely low.

To optimize the search for new fluorescent proteins (FPs), a technique was developed that allows for the rapid cloning and screening of FP genes without the need for a prior knowledge of gene sequence.

Another major advantage of this new methodology is the ability to express integrated genes without the need for maintaining antibiotic selection, making this an ideal tool for functional studies of genes in infection models and co-culture systems.

To generate insulin-producing cells in the liver, recombinant adenovirus containing a constitutively active mutant of PDX1 (Pdesigned), designed to activate target genes without the need for protein partners, was prepared and administered intravenously to streptozotocin (STZ -treated diabetic mice.

This strategy successfully enlarged the number of detected genes without compromising the specificity of the detection.

Importantly, changing the mitochondrial background alters the effects of the interacting nuclear genes without changing the nuclear gene sequence.

Weigel et al. (2000) reported that the 35S enhancer element might increase the expression of nearby genes without altering the original expression pattern.

Epigenetic alterations are chromatin-based modifications that affect the expression of genes without altering the DNA sequence itself.

Exposure to harmful substances in the environment can cause mutations in egg or sperm cells, or alter the expression of genes without changing the gene sequence.