Sentence examples for genes with optimal from inspiring English sources

We have optimized the expression of several far-red fluorescent reporters in M. tuberculosis by combining codon-biased synthetic genes with optimal promoters and replicons to produce reporter strains that can be detected with great sensitivity in vitro.

Encoding genes with optimal codons might not always lead to higher protein expression levels (Kudla et al., 2009).

The JCat tool uses the method of Carbone and colleagues [ 44] to select a set of reference genes with optimal codon usage.

In support, Springer and Stupar [ 43] argued that many genes with optimal expression ranges are known, citing as examples genes involved in pathogen defense [ 44].

While gene expression profiles tend to be more robust with inclusion of a larger set of genes [ 9], we decided to limit the developed profile to a relatively small number of genes with optimal performance.

Since the order of fusion genes in TF determines the mRNA structure that may influence ribosomal accessibility or mRNA stability, we aimed to construct a new TF reporter gene with optimal configuration for multimodality imaging, the p3H (DsRedm-fl-ttksr39), and to compare this novel TF gene with other two TF genes, the p3G (rl-DsRedm-ttksr39) and p3R (fl-DsRedm-ttksr39).

It is time to start including genetic markers in the daily risk assessment in UTI-prone patients to validate genes or gene combinations with optimal predictive power in the different UTI-prone patient groups.

Since RT-PCR is still considered the "golden standard" for gene expression measurements, we further used SYBR green-based quantitative real-time PCR to validate the relative gene expression for a few of the 1478 additional genes identified with optimal conditions.

The explanation may be that the negative correlation between optimal codon use and gene length simply is a side effect: highly expressed genes with high optimal codon use tend to be short, possibly to be more economic [ 19].

We wished to determine whether highly expressed genes with elevated optimal codon usage were preferentially associated with particular functions, biological processes or cellular components.

Full-length human wild-type and mutant (p.V432fsX87) WT1 genes with an optimal Kozak sequence in front of the start ATG were cloned through PCR from mutant WT1 Wilms3++ and WT1 Wisoformssoforms in pCRII-TOPO vectors and from wild-type WT1++ and WT1+− isoforms in pDNA3.3-Topo-TA vectors.