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The gene sequence showed a close homology to fungal β-tubulin genes with four introns.
These loci were filtered further and divided into 39,734 high-confidence genes (with four different sub-categories) and 41,949 low-confidence genes on the basis of sequence homology to related species (Methods and Supplementary Note 3.4).
Both CD7 and Thy-1 are encoded by small genes with four exons, contain TATA-less promoters, and have a similar functional organization.
Yet, scientists don't know why DNA spells out genes with four chemical letters instead of two or six.
We simulate networks of size 50, 100, 200, and 500 genes with four types of perturbations.
In addition, there are three other WP3 specific genes, with four copies each, involved in sulfur metabolism (Supplementary Table S8).
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Of 25,559 mapped genes, 2,440 genes with zero counts across all subjects were excluded.
For genes with two probes, the one with the highest variance was selected.
Table 1 Genes with three or more SNVs in the B. cenocepacia sequential isolates.
There were 12 genes with three or more mutations (Table 1) which has a zero probability of occurring by chance29.
Accordingly, we selected genes with two or more shRNAs with a fold change (aurilide B-treated/untreated) <0.4 (Experiment 1) or 0.33 (Experiment 2).
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