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Genes with binding motifs for Ca++ may bind other divalent cations, and zinc binding has been shown to confer anti-microbial actions to S100A7 [27] and the S100A8/S100A9 (calgranulin) complex [28].
Applying chromatin immunoprecipitation coupled with deep sequencing (ChIP-Seq) in the human SGBS pre-adipocyte cell line, we identified genes with binding sites in their vicinity for the three TFs studied, PPARγ, CEBPα and LXR.
Those downregulated genes with binding sites for at least 2 out of the 6 selected microRNAs were then subjected to pathway analysis at http://david.abcc.ncifcrf.gov/tools.jsp [35].
In other words, as N increases, the number of genes with products corresponding to a binding site of gene i increases (in-degree), and the number of genes with binding sites corresponding to the product of gene i increases (out-degree).
Therefore, equivalent analyses were performed after excluding genes with binding sites of multiple categories.
The network is constructed by connecting genes with binding sites if their binding specificity is equal.
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To illustrate the difference of these two cell lines, we first compared the overlapped number of genes with ERα binding peak and Pol-II binding peak, respectively.
Notably, we observed substantial decrease of expression levels of genes associated with binding of EZH2 while the accessibility level showed just a moderate change (Fig. 1e).
Genes associated with binding (i.e. recognising) chitin, a primary component of fungal cell walls and insect exoskeletons, and chitinases, enzymes designed to degrade chitins, were up-regulated in the xylem.
The molecular function category was rich in genes associated with binding (GO ID: 0005488), catalytic activity (GO ID: 0003824), and structural molecules (GO ID: 0005198).
Gene ontology (GO) analysis of the genes with constitutive binding sites in their promoters suggests that constitutive binding sites for several of the proteins are biologically meaningful.
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