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Expression levels of a subset of those genes were verified using quantitative RT-PCR (Fig. S1).
A total of 108 non-synonymous somatic mutations (NSSMs) in 102 genes were verified in nine patients.
Expression changes of 17 selected genes were verified and an additional 19 genes were examined by real-time quantitative PCR.
However, by 25 h, 128 genes were differentially expressed and expression patterns of a subsample of these genes were verified using RT-qPCR.
The transcription levels of a selected pool of differentially regulated genes were verified by qPCR.
Finally, the correct nucleotide sequences of the native gene and all mutated genes were verified.
Microarray expression profiles of selected genes were verified with real-time qPCR and immunoblotting.
Both genes were verified to regulate biofilm formation through gene knockout experiments.
The coding sequences of all the recombinant genes were verified by DNA sequencing.
Finally, the correct nucleotide sequences of the native gene and all truncated genes were verified.
Knockdowns of individual genes were verified by RT-PCR (data not shown).
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