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The RNA-seq results of selected genes were validated by real-time RT-PCR.
Tolerance-specific genes were validated in independent samples across two different transplant programs and validated by Q-PCR.
MTH-68/H-induced gene expression changes of 9 genes were validated using quantitative reverse transcriptase PCR.
A group of the cardiac-enriched genes were validated by qRT-PCR (Fig. 2B).
The expression patterns of twelve candidate genes were validated using the same RNA samples as in the microarray analysis.
Differential expression patterns for select genes were validated with quantitative reverse transcriptase PCR (qRT-PCR).
The changes in transcript abundance of selected genes were validated by quantitative RT-PCR (Figure S1).
Lastly, as proof-of-concept, 5 specific genes were validated for direct regulation.
Selected genes were validated with qRT-PCR.
Afterwards, selected genes were validated by qPCR.
Screened miRNAs and genes were validated using quantitative qPCR.
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