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These genes were subsequently cloned, sequenced, and characterized.
More than 30 Ir genes were subsequently found, and that genetic material was determined to be part of the major histocompatibility complex, a complicated region of DNA involved in immune responsiveness.
The amplified genes were subsequently cloned into the pCWBM3 BamHI/SacI vector at the BamHI/SacI restriction sites.
These genes were subsequently found in humans and other mammals as well.
Genes were subsequently identified in the remaining unique sequences using Glimmer3 [54].
All the responsive genes were subsequently validated by RT-PCR (Fig. 1B).
Primer pairs for these genes were subsequently designed with the Primer3 software [50].
All the remaining exons in 11 of the 15 genes were subsequently sequenced.
It was of further interest to determine how these two categories of genes were subsequently affected by DHT treatment.
The 51 fetal liver-specific genes, and 50 trophoblast-related genes were subsequently analyzed for detectability in blood.
Both myc-His tagged CSNK2A1 and CSNK2A1P genes were subsequently cloned into the SnaBI site of the pBabe-puro vector.
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