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The genes were removed efficiently from the genome of P. mendocina NK-01 and left a markerless chromosomal mutant.
All probe-sets annotated as recognizing multiple genes were removed.
These genes were removed from the final analysis.
Therefore, these genes were removed from the study.
These genes were removed from the dataset and a further set of classifiers were obtained.
Quality control probesets (i.e., spike in and housekeeping genes) were removed in subsequent statistical analyses.
Next, complex and monogenic disease genes were removed from that list resulting in 1986 essential genes.
The corresponding genes were removed from the tagged gene list before performing comparative genomic analyses.
Approximately 10 20% of the genes were removed in this way.
Redundant genes were removed manually.
The ribosome-related genes were removed as previously described.
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