Sentence examples for genes were quantified from inspiring English sources

Subsequently the cells were treated with 1 μg/ml of Actinomycin D for the indicated times to block de novo transcription and relative mRNA levels of CNOT2 target genes were quantified by RT-qPCR anormalizedzed to the Actin levels.

The mRNA expressions of chondrogenic (SOX-9, aggrecan and collagen type II), fibrocartilage (collagen type I), and hypertrophic (collagen type X) marker genes were quantified by real-time polymerase chain reaction.

Abundances of transcripts of target genes were quantified by quantitative real-time reverse transcription PCR (qPCR).

In addition to expression of cyp1a, abundances of transcripts of several other genes were quantified.

Normalised expressions of the AmSesTPS1 and AmGuaiS1 genes to the housekeeping genes were quantified in the 54 samples using the same batch of cDNAs to minimise experimental variation.

The genes were quantified in triplicate.

Genes were quantified using only the single-best mapping methodology.

For both types of gene expression the target genes were quantified by relative quantification using the comparative CT method.

Standard curves with four dilutions were made in duplicates for each primer pair to calculate the amplification efficiency, and all genes were quantified in triplicates.

To validate the microarray results, mRNA levels for a subset of genes were quantified by quantitative real time PCR (qRT-PCR) (Fig. 2).

The three genes were quantified in the DNA isolated from bacteriophages collected from 30 urban sewage and river water samples, using quantitative PCR amplification.