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The amplified functional genes were purified with a gel extraction kit (Himedia, India) according to manufacturer's instructions.
The PCR amplified products of cloned aioA genes were purified using the Wizard PCR SV Gel and PCR Clean-Up System kit (Promega, USA).
The PCR products of all target genes were purified with the EZNA Cycle-Pure Kit (Omega, USA) and digested with Nco I and Xho I.
These resulting fragments containing the homologous recombination regions and the hexon genes were purified, then recombined with a SwaI-digested Ad5 backbone vector that lacks the hexon gene, pAd5/ΔH5 [26].
The targeted genes were purified by CWBIO Gel extraction kit.
Prior to splicing, the amplified genes were purified using a QIAquick PCR purification kit (QIAGEN, Germany).
Similar(41)
In brief, a BAC (bacterial artificial chromosome) clone named BAC RP11-466J14, whicontainsins the CYP2C18 and CYP2C19 genes was purified.
The PCR product generated for both F and G genes was purified with the QIAquick gel extraction kit (Qiagen, Inc).
PCR products of 1.8 kb (phytoplasma 16S rRNA gene) and 0.8 kb (tuf gene) were purified using a commercial PCR Purification Kit (Bio Teke Corporation, Beijing, China) and cloned.
The PCR amplicons of 16S rRNA gene were purified with a Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) and condensed to a final volume of 20 μl in sterilized-MilliQ water.
Plasmids containing the target gene were purified and quantified by UV spectrophotometry.
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