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Additionally, probes for the DNA methyltransferase genes were produced to determine if gene dosage alterations were responsible for increasing methylation activity during Barrett's neoplastic progression.
Viral particles carrying the receptor genes were produced by three plasmid transfection, as previously described [42].
Three separate replication-defective rAd serotype 5 vectors expressing HA (KAN-1), NP (KAN-1), and M2 (KAN-1) genes were produced as previously described [23].
For preparation of SW480/ABCG2, retrovirus encoding ABCG2 genes were produced by using BglII/XhoI sites.
P labeled probes of selected genes were produced with the High Prime System (Roche Diagnostics).
Multiple alignments of genes were produced with the ClustalW algorithm implemented in MEGA 4 [ 90, 95].
Similar(37)
Additionally, a recipient strain of Synechocystis lacking all three psbA genes was produced by a combination of traditional marker replacement and markerless replacement techniques.
This last strategy, opposite to the first one, suggest a transient necessity of these processes, whose genes are produced at a fast rate but quickly discarded after their functions have been carried out.
Most protein coding genes are produced by gene duplication followed by neofunctionalisation and/or subfunctionalisation.
Some genes are produced 1.5 times more than usual, but others are reduced [ 20, 35, 36].
A UniGene set of 2800 genes was produced from a cold induced oat cDNA library.
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