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Approximately 2.5% of the identified genes were previously shown to play a role in the HIV-1 life cycle; this finding supports the biological relevance of the assay.
The normalization genes were previously tested and the gene with the least variation between samples was chosen.
Primer sets corresponding to different fusion genes were previously described and are listed in Table 1 [10,16].
These genes were previously reported to be upregulated slightly under −P stress based on qRT-PCR analysis and northern analysis (Rubio et al. 2001; Miura et al. 2005).
The upregulated genes were previously reported in studies on drought, salt and other abiotic stresses, but not with respect to submergence stress (Additional file 2: Table S7).
Several photosynthesis- and Chl synthesis-associated genes were previously found to be downregulated in the green sectors of 2-week-old fgl leaves under CL conditions (Sakuraba et al. 2013).
Despite having been the subject of intensive genetic analysis for about 40 years, a large number of these genes were previously unknown.
The genes were previously predicted from the genome deposited in GenBank accession number AATU01000000 [11].
Primers used for Dr V1r2, β-actin and OMP genes were previously described [16].
These genes were previously correlated to cancer cell invasion and metastasis.
Both genes were previously codon-optimized for expression in E. coli [40].
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