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Only 315 genes were picked in both FF and FFPE samples, and all these 315 were also picked-up in the combined analysis.
However, the top five genes were picked from the combined cohort.
36 up-regulated genes and 356 down-regulated genes were picked out (See Additional file 2).
Differentially expressed genes were picked using SAM [ 85] (Two class Paired).
Therefore, the top 11 proteins (11 genes) were picked out for HRV (betweenness > 47,299) while the top 44 proteins (44 genes) were picked out for RSV (betweenness > 1,275,672) from the lists in Supporting Information S4, respectively.
Also, these genes were picked up for additional in-depth study because they are involved in different cellular functions.
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According to this hypothesis, candidate genes are picked from high-throughput data on the basis of their biological proximity to core genes — genes already known to be responsible for the phenotype.
At least three independent isolates for each gene were picked and cultured overnight in 2 ml Luria Broth (LB) containing 25 µg/ml kanamycin and 25 µg/ml chloramphenicol.
Thus, the complexity of crossover stage is O(N c P Size ). (6) Mutation: because the uniform mutation is adopted and the mutated gene is picked randomly from the chromosome, the mutation operator needs N c P Size steps to fill overall populations.
Where all PSM from a given HCMV protein could be explained either by a canonical gene or noncanonical ORF, the canonical gene was picked in preference.
Where all PSMs from a given HCMV protein could be explained either by a canonical gene or novel ORF, the canonical gene was picked in preference.
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