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Plasmid DNAs carrying luciferase, β-galactosidase (β-gal), or enhanced green fluorescent protein (EGFP) reporter genes were mixed with BR14 and injected percutaneously into the left ventricular (LV) cavity of C57BL/6 mice while exposed to transthoracic ultrasound at 1 MHz for 60 s.
Briefly, 2 μL of customized reverse primers of the desired genes were mixed with 11 μL of RNA free water, 4 μL of reverse transcription buffer, 1 μL of reverse transcriptase and 1 μL of 50 ng/μL of sample.
Colonies of specific genes were mixed into different pools of defined clone compositions and the detection width was adjusted to be the best compromise between not missing any real hits and counting false ones.
Primer sets specific to the lambda integrase and T4 bacteriophage gp23 major capsid genes were mixed and used in gradient PCR to identify the annealing temperature for subsequent reactions (Table S1).
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It is interesting to note that the top univariate genes all have higher expression levels for symptomatic patients, while the BCell+CAM genes are mixed.
The 5′-end of the RefSeq genes was mixed with 50-kb upstream regions to remove the possible alternative promoters of the RefSeq genes (Supplementary Fig. S1E).
Plasmid DNA containing the hwtSOD1 gene was mixed with plasmid DNA containing a mutant SOD1 gene (hSOD1 E77X, or hSOD1 K91X ) in equal amounts (2 µg each).
Briefly, for the live-imaging analysis, a library targeting the human actinome, four siRNAs per gene, was mixed with a transfection reagent and arrayed in spots onto glass chamber slides [ 11].
An expression plasmid carrying the cytomegalovirus enhancer/chicken β-actin promoter linked to a DT-A gene was mixed with lipid (FuGENE™6) and the resulting complexes were intravenously injected into adult male B6C3F1 mice every day for up to 6 days.
Both gene probes were mixed their relevant reference probes in the IQFISH Buffer (Dako) [ 19].
In a series of simulation experiments, known functionally coherent gene sets were mixed and recovered using our approach.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com