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Both genes were isolated and expressed in Escherichia coli.
NAC-type transcription factors that regulate expression of abiotic stress responsive genes were isolated initially from Arabidopsis.
To find genes that play roles in initial regionalization of anterior neuroectoderm, 15 novel genes were isolated that are expressed in anterior neuroectoderm at E8.0 E8.5.
In this paper, 12 Qc-SNARE genes were isolated from rice, and expression patterns of 9 genes were detected in various tissues and in seedlings challenged with oxidative stresses and inoculated with rice blast.
Both the promoterless genes and the promoter-bearing genes were isolated through PCR amplification with properly designed primers, and were inserted into plasmid pSG703 that contained the lacZ-cat expression cartridge.
Fragments of these genes were isolated by PCR.
Three full-length cDNA clones, homologous to Arabidopsis PRR genes were isolated.
The cyt b and COI genes were isolated from the complete sequences for alignment.
The polyadenylation regions of the human β−globin (HBB, NM_000518) and β−actin (ACTB, NM_001101) genes were isolated using PCR.
The genes were isolated and sequence characterised in B. betularia using degenerate PCR and from whole-transcriptome sequence.
Matings were performed at room temperature or 37°C for 3 hours or overnight but no transconjugants carrying island genes were isolated.
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