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All 55 pairwise combinations of the 11 top RNAi target genes were injected into larvae at the same end concentration as the single injections (i.e. either 0.5 ng/μl of one dsRNA or 0.25 ng/μl of two dsRNAs summing up to 0.5 ng/μl end concentration) and the survival was documented (Additional file 1: Table S4).
The fused genes were injected into tobacco and the fluorescent signal was detected after transformation.
Modified BACs containing inserted E-GFP upstream of targeted genes were injected into pronuclei of FVB/N fertilized mouse oocytes.
The antisense morpholinos (MOs) targeting SerRS or other genes were injected into the yolk of 1- to 2-cell stage embryos at the dosage of 4 ∼ 5 ng per embryo.
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Three days before mosquitoes were fed with dengue-infected blood, double stranded RNA (dsRNA) of each of these genes was injected into the thorax of mosquitoes.
It will be exciting to investigate whether or not any of these genes are injected into the host during tick feeding.
After 10 days, 1×106 iPS-MSCs transduced with the CD gene were injected into the tumors or adjacent tissue.
For the Nalm6 leukemia model, 1 × 106 Nalm6-CBG cells (Nalm6 transduced via lentivirus with the click beetle green luciferase gene) were injected into each mouse via the tail vein.
Recently, mdx iPSCs and ESCs containing an artificial chromosome with a full-length human dystrophin gene were injected into mdx blastocysts to supply human dystrophin to muscle and heart, but corrective analyses of the muscle were not performed [36], [37].
Nanoparticles containing the wild-type retinal degeneration slow (Rds) gene were injected into the subretinal space of rds+/− mice on postnatal day 5. Gene expression was sustained for up to four months at levels up to four times higher than in controls injected with saline or naked DNA.
In addition, 15/16 of the phenotypes in Table 1 were reproducible when two sequence-independent MOs were tested and, furthermore, these demonstrated synergistic effects when both MOs against the same target gene were injected together (criteria for specificity as in[5], [6]).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com