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Thus, two codon-optimized PfAQP genes were generated.
A large number of rodent carboxylesterase genes were generated from tandem gene duplication.
Stable strains expressing each of the genes were generated and cultured in the absence or presence of selenocystamine increasing concentrations.
These fused genes were generated by overlap extension PCR, and the linker sequence between them was 5′-CTGGACAGCACC-3′.
The mutated crp genes were generated via error-prone PCR and three toluene-tolerant mutants M1 M3 were isolated from random mutagenesis libraries by enrichment selection.
Primer sequences specific to 12 of target genes were generated with Beacon Designer software (Bio-Rad).
Clones for the transient and transgenic expression of Arabidopsis genes were generated using Gateway technology (Invitrogen).
Probes 1 11 of the candidate genes were generated from E15 whole mouse RNA.
The lists of differentially expressed genes were generated and passed through GOstats for pathway analysis.
In order to determine the role of the LS genes, simple and double mutants for the ribH genes were generated.
Views of individual genes were generated by uploading coverage.wig files to the UCSC Genome browser as a custom track.
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