Therefore, expression levels of BR signaling genes were further analyzed.
Plasmids containing the mutated genes were further transformed in E. coli DH5α following the manufacturer protocol.
Based on the expression profiles, 19 genes were further analysed by qPCR.
Novel genes were further characterized by tissue-specific patterns, cellular localization, and chromosomal location.
The newly identified genes were further filtered using a permutation test.
The new DHQase genes were further customized, by linking to Biobrick adapters (Knight 2003) at both ends.
Therefore, the expression patterns of these genes were further investigated in leaves and young panicles from HMK and TN1 varieties.
To validate the results, five differentially expressed genes were further examined by quantitative real-time PCR (qPCR).
The annotated putative genes were further explored for their known role in defense mechanisms including disease resistance.
In all studies, these genes were further upregulated at day 7. Upregulation of osteogenic factors seemed regulated by implant surface.
A total of 4368 annotated genes were further processed.
