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The selected candidate reference genes were evaluated by qRT-PCR with the ΔCt approach.
The expression stability of these candidate reference genes were evaluated using the four methods described below.
These genes were evaluated through qRT-PCR experiments with a rigorous statistical analysis to determine the best reference genes.
Diverse expression patterns for the sixteen genes were evaluated using both semi-quantitative RT-PCR and the MPSS data.
Informative genes were evaluated in the 37 remaining patients and ATP1A3 emerged as the gene associated with AHC [3].
The housekeeping genes were evaluated for their expression stability in samples from plants subjected to different forms of abiotic stress.
The ABL with three resistance genes were evaluated for their resistance to BB under glasshouse conditions with the 18 isolates of Xoo prevalent in Korea.
They were incubated with RAW264.7 cells, and changes in the expression of genes were evaluated by cDNA microarray analysis and quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR).
The effect of different conformation of collagen on cell adhesion, morphology, proliferation, alkaline phosphatase activity and expression of three osteoblast-related genes were evaluated.
A previously designed end-point multiplex PCR assay and singleplex assays used to detect β-lactamase genes were evaluated using rapid PCR amplification methodology.
To monitor the ability of GTE to counteract the apoptotic effects induced by CNPs, DNA fragmentation percentage, DNA laddering assay, and the expression of some apoptotic genes were evaluated.
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