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After activation, transcript amounts of individual genes were enumerated by quantitative RT-PCR.
After these incubations, transcript amounts of individual genes were enumerated by quantitative RT-PCR.
In order to deal with this possibility, the genes were enumerated on the basis of the positions of their p-termini.
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Among these 64 genes, 40 were induced>2.0-fold by UVA/UVB, these genes are enumerated in Table 1.
The gene expression levels as measured by the relative quantification method for our study genes are enumerated in Table 2. Gene expression levels are relative to GAPDH expression where GAPDH expression level is equal to 1.
The genes are enumerated in additional file 1. Endogenous control genes are required to account for the amount of input RNA, and they need to be equally expressed across illness class and time points.
The methylation status and expression levels with respect to the study genes are enumerated in Table 3. TNF methylation was examined at the promoter region and the first exon.
Mate-pair reads separated by more than one standard deviation from the mean fragment size were identified, and those mate-pairs containing one read in an ERV located upstream of the first exon of a gene, and the other read in an annotated genic exon of that gene, were enumerated.
After induction of both the TA genes, culturable bacteria were enumerated after plating on to agar.
The efficacy of the DNA microarray was verified in a parallel study where pathogen genes and E. coli cells were enumerated using real-time quantitative PCR (qPCR) and standard membrane filter techniques, respectively.
The length of chromosome 7 was subdivided into a sequence of non-overlapping and equal-sized bins, and the gene structures within each bin were enumerated.
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