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PCRs for Cyp2c genes were done without SYBR Green but with fluorescent labelled probes.
Here, phylogenetic analysis and chromosomal location of genes were done to trace the evolutionary history of the CYP3 family in Actinopterygii.
Comparisons between genes for the identification of common genes were done using BLAST similarities of e-value 10−5 and similarity >20%.
Local investigations of the MTAP, CDKN2A, CDKN2B and ANRIL genes were done using the GeneRegionScan package [14] from the Bioconductor repository [18] to extract probe level data and de novo annotate each probe to its location along the length of the gene.
The original data reporting association between the CCL2 and TIRAP genes were done by Ramasawmy et al.[ 41, 42].
PCRs targeting outer membrane protein (omp) A and B genes were done by using previously described primers (3 ).
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Classification of genes was done using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database (http://www.genome.jp/kegg/).jp/kegg/
The selection of all genes was done in one generation, which could not be achieved through phenotypic selection.
Functional characterization of both the genes was done through enzyme assays and tissue-specific expression of both the genes was studied.
In the present study homology analysis of these genes was done in order to know its phylogenetic relationship among other bacteria/mycobacteria.
SamTools mpileup command was used to generate consensus sequences against the reference for each gene and alignment of OsHKT genes was done by ClustalW (Thompson et al. 1994) in BioEdit (Hall 2011).
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