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Short genes were discarded using the same filter threshold as for homology prediction.
Adjacent segments and segments separated by no more than 1-Mb were merged together, while segments containing no more than five genes were discarded.
GO terms and domains with less than 4 genes were discarded.
Probe sets that mapped to more than two different Ensembl genes were discarded.
Probe sets that do not map to any HUGO genes were discarded.
Only probe-sets that identified unique genes were utilized in this analysis and all probe-sets that identified multiple genes were discarded to control for potential cross-hybridization.
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During the evolution of a genome, those genes required for or that facilitate the survival of a species are created and maintained, whereas unwanted genes are discarded.
Usually, transferred genes are discarded before gene concatenation [ 23, 24].
Probesets mapping on multiple genes are discarded from the calculations.
Single-exon genes are discarded at this point.
An EST matched to multiple genes was discarded.
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